There are 4 Key Seals in the lands of La-Mulana. They are used to open passageways, reveal hidden areas, or complete puzzles by breaking Wall Seals with the same symbol.
It is necessary to obtain all 4 to finish the game.
 Conception Seal
- Location: Spring of the Sky E-2
- Defeat the Jackrabbit at E-3.
 Birth Seal
- Location: Surface L-2
- Jump from L-1 onto the floating platform at L-2. From there leap over to the right side of the screen and use the Conception Seal.
 Life Seal
- Location: Chamber of Extinction F-4
- Break the left wall of the green pool at H-5 and move left through the hidden passages to reach F-5. Push the block on to the designated area to open the chest.
] [[Death Seal]
- Location: Shrine of the Mother A-3
- Use Bombs to break the column that hangs over the chest and reveal a pedestal. You must visit the four sages with the Ocarina to make the ladder appear in the Endless Corridor.
- If the shrine has already been transformed you can still obtain this by double jumping though the tentacle up to the seal.